Relative Evaluation of the Application of Polystyrene Microspheres and Polystyrene Carboxyl Microspheres in Biotechnology – Focusing on Nucleic Acid Extraction.
(LNJNbio Polystyrene Microspheres)
In the field of modern-day biotechnology, microsphere products are widely used in the extraction and filtration of DNA and RNA due to their high particular surface, good chemical stability and functionalized surface properties. Amongst them, polystyrene (PS) microspheres and their derived polystyrene carboxyl (CPS) microspheres are among both most extensively studied and applied materials. This article is given with technical assistance and information analysis by Shanghai Lingjun Biotechnology Co., Ltd., aiming to systematically compare the performance distinctions of these 2 types of materials in the process of nucleic acid extraction, covering vital signs such as their physicochemical buildings, surface modification ability, binding efficiency and recovery rate, and highlight their applicable circumstances with speculative data.
Polystyrene microspheres are homogeneous polymer particles polymerized from styrene monomers with great thermal stability and mechanical strength. Its surface area is a non-polar structure and normally does not have active useful teams. Consequently, when it is directly used for nucleic acid binding, it needs to rely upon electrostatic adsorption or hydrophobic action for molecular fixation. Polystyrene carboxyl microspheres introduce carboxyl functional teams (– COOH) on the basis of PS microspheres, making their surface area with the ability of more chemical coupling. These carboxyl teams can be covalently bonded to nucleic acid probes, healthy proteins or other ligands with amino teams via activation systems such as EDC/NHS, consequently achieving more stable molecular addiction. As a result, from a structural point of view, CPS microspheres have a lot more advantages in functionalization potential.
Nucleic acid removal generally consists of steps such as cell lysis, nucleic acid launch, nucleic acid binding to strong stage providers, washing to get rid of contaminations and eluting target nucleic acids. In this system, microspheres play a core role as solid stage providers. PS microspheres mainly rely on electrostatic adsorption and hydrogen bonding to bind nucleic acids, and their binding performance is about 60 ~ 70%, yet the elution performance is low, just 40 ~ 50%. On the other hand, CPS microspheres can not only utilize electrostatic impacts however additionally achieve even more strong fixation via covalent bonding, decreasing the loss of nucleic acids throughout the washing procedure. Its binding effectiveness can get to 85 ~ 95%, and the elution performance is also enhanced to 70 ~ 80%. In addition, CPS microspheres are likewise considerably far better than PS microspheres in regards to anti-interference ability and reusability.
In order to verify the performance distinctions in between the two microspheres in real procedure, Shanghai Lingjun Biotechnology Co., Ltd. performed RNA extraction experiments. The experimental samples were originated from HEK293 cells. After pretreatment with common Tris-HCl barrier and proteinase K, 5 mg/mL PS and CPS microspheres were utilized for extraction. The results revealed that the average RNA return drawn out by PS microspheres was 85 ng/ μL, the A260/A280 ratio was 1.82, and the RIN value was 7.2, while the RNA return of CPS microspheres was raised to 132 ng/ μL, the A260/A280 ratio was close to the ideal worth of 1.91, and the RIN worth got to 8.1. Although the operation time of CPS microspheres is a little longer (28 mins vs. 25 mins) and the price is greater (28 yuan vs. 18 yuan/time), its extraction top quality is considerably enhanced, and it is more suitable for high-sensitivity discovery, such as qPCR and RNA-seq.
( SEM of LNJNbio Polystyrene Microspheres)
From the point of view of application scenarios, PS microspheres appropriate for large-scale screening tasks and preliminary enrichment with reduced demands for binding uniqueness due to their affordable and simple procedure. Nonetheless, their nucleic acid binding capacity is weak and quickly impacted by salt ion focus, making them unsuitable for long-term storage or repeated use. On the other hand, CPS microspheres appropriate for trace sample removal as a result of their abundant surface area practical teams, which assist in more functionalization and can be used to build magnetic grain detection sets and automated nucleic acid removal platforms. Although its prep work process is relatively complex and the expense is reasonably high, it shows more powerful flexibility in scientific research and medical applications with stringent requirements on nucleic acid extraction efficiency and pureness.
With the rapid development of molecular medical diagnosis, gene modifying, liquid biopsy and other areas, higher demands are placed on the performance, purity and automation of nucleic acid removal. Polystyrene carboxyl microspheres are progressively changing conventional PS microspheres as a result of their outstanding binding performance and functionalizable attributes, coming to be the core selection of a new generation of nucleic acid extraction products. Shanghai Lingjun Biotechnology Co., Ltd. is likewise continually enhancing the particle dimension circulation, surface area thickness and functionalization efficiency of CPS microspheres and developing matching magnetic composite microsphere items to fulfill the demands of scientific diagnosis, scientific research study institutions and commercial clients for top quality nucleic acid extraction remedies.
Provider
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding and more. We not only provide products but can also undertake OEM, ODM, and other needs. If you need extraction of rna, please feel free to contact us at sales01@lingjunbio.com.
All articles and pictures are from the Internet. If there are any copyright issues, please contact us in time to delete.
Inquiry us
